SELECTIVE SPECTROPHOTOMETRIC QUANTIFICATION OF IPRIFLAVONE IN PRESENCE OF ITS DEGRADANTS


Ezzat M. Abdel-Moety, Soheir A. Ahmed, Samah S. Abbas and Sherif A. Abdel-Gawad

Pharmaceutical Analytical Chemistry Department ,Faculty of Pharmacy - Cairo University ,Kasr El-Aini Post, ET-11562 Cairo – Egypt

Received:25-11-2008

Accepted:28-12-2008




Abstract



Derivative spectrophotometric methods are described for selective determination of ipriflavone (IPR) in pure form and in tablet, singly or in presence of its acid, alkaline & oxidative degradants. Quantification of intact ipriflavone in presence of ~ 80% of its acid degradants could be done by first derivative spectrophotometry (1D) at 319.2 nm with mean percentage recovery of 100.04+0.300, as well as, by first derivative of the ratio spectrophotometry (1DD) at 319.6 nm utilizing the two acid-degradants separately as divisors, with mean percentage recovery of 99.93 + 0.320 & 100.05 + 0.290. Second derivative (2D) spectrophotometry at 324.4 nm and 1DD spectrophotometry at 269 nm & 319.6 nm are demonstrated too for determining the drug in presence of up to 80-90% degradants.  Quantification of intact IPR in presence of its alkaline-degradant was undertaken by 3rd derivative spectrophotometry (3D) at 268 nm & 318.8 nm, with mean percentage recoveries of 100.16+0.670 & 100.13+ 0.740, respectively, and 1DD spectrophotometry at 269.6 nm & 320.6 nm, with mean percentage recoveries of 100.09+0.780 & 99.85 + 0.750, respectively. IPR could be accurately quantified in presence of ~ 80% & 90% of its oxidative derivative by 2D and 1DD-spectrophotometry at 314.7 nm - 324.5 nm and 253 nm & 320 nm, respectively, with excellent percentage recoveris.

Keywords: Spectrophotometry; Ipriflavone; Stability-indication; Determination.